Droplet Insider vol. 2 – Droplet-microfluidics-assisted transcriptome sequencing of the HIV-Infected ‘Latent Reservoir’

We are honored to have Dr. Eli Boritz as the next speaker in the second volume of Droplet Insider.

Dr. Boritz and his team recently published a paper in Nature, which spotlights their application of a novel method to sort and analyze subsets of cell types based on RNA or DNA biomarkers. The team used this method to study mechanisms of HIV persistence under antiretroviral therapy, particularly the expression profiles of HIV-infected memory CD4 T cells.

The method, Focused Interrogation of cells by Nucleic acid Detection and Sequencing (FIND- seq), involves identifying cells by target nucleic acids (HIV in this study) and sorting them for single-cell resolution transcriptomic analysis.

The authors used microfluidics to create agarose gel beads to encapsulate the genetic material of individual cells and sorted the agarose beads based on the fluorescent signal of the HIV nucleic acid marker. The whole transcriptomes of cells are retained inside the agarose beads and can be analyzed by sequencing later.

The transcriptomes of HIV DNA+ memory CD4 T cells from people receiving antiretroviral therapy showed a distinctive population with host gene expression patterns that favour HIV silencing, cell survival and cell proliferation, with important implications for the development of HIV cure strategies.

What you will learn:

• A novel method for RNA-sequencing in cell subsets, defined by RNA or DNA biomarkers.
• Application of FIND-seq in studying the transcriptomes of HIV-infected memory CD4 T cells.
• Findings in mechanisms of HIV persistence under antiretroviral therapy.

Droplet Insider vol 1: Microfluidics for Biology (US session)

In life sciences, microfluidics is used to study biological processes at the cellular and molecular level. Microfluidics has previously been used in the study of single cells, vaccine development, and drug discovery. It has advantages in “the use of small volumes and precise liquid handling, which enable cost-effective high-throughput biochemical assays and diagnostics” (Ortseifen et al.)

Nevertheless, the questions on what microfluidics technology is, what it can do and how to get started with a microfluidics experiment for a new application are not often discussed among biologists. In this webinar, we’ll share with you about the fundamentals of microfluidics and how the technology can be used for different biology experiments. A 10-mins demo on our Nadia platform will also show you how easy it is to start your first microfluidics experiment with your current lab settings.

What you will learn:

• Why is microfluidics a useful/ essential tool for biologists?
• Microfluidic solutions for current demands in life science research
• How to start your first microfluidics experiment

Droplet Insider vol 1: Microfluidics for Biology (APAC session)

In life sciences, microfluidics is used to study biological processes at the cellular and molecular level. Microfluidics has previously been used in the study of single cells, vaccine development, and drug discovery. It has advantages in “the use of small volumes and precise liquid handling, which enable cost-effective high-throughput biochemical assays and diagnostics” (Ortseifen et al.)

Nevertheless, the questions on what microfluidics technology is, what it can do and how to get started with a microfluidics experiment for a new application are not often discussed among biologists. In this webinar, we’ll share with you about the fundamentals of microfluidics and how the technology can be used for different biology experiments. A 10-mins demo on our Nadia platform will also show you how easy it is to start your first microfluidics experiment with your current lab settings.

What you will learn:

• Why is microfluidics a useful/ essential tool for biologists?
• Microfluidic solutions for current demands in life science research
• How to start your first microfluidics experiment

Hydrogels, Microfluidics, and Cell Biology – Session 1

One of the greatest difficulties faced by researchers interested in using hydrogels for single cell research is the lack of a reliable, standardized, high throughput, reagent kit that is available commercially. To enable you to explore this rapidly developing area of research with ease, Do Bio has developed two hydrogel reagent kits – one is designed to address the needs of those interested in agarose encapsulation, the other is a collagen-based kit, developed for the 3D culture space.

In this webinar, you will learn about the benefits of using hydrogel kits for the production of agarose and 3D cell scaffolds using the Nadia Instrument. We will also cover the details of our simplified Nadia workflow that offers reliable and reproducible results, which will help you construct the future of single cell research.

This webinar will cover: 
– The benefits of using reagent kits
– Applications for large hydrogel scaffolds
– Applications for small hydrogel scaffolds

Getting started in single cell research with the RNAdia data analysis service

Getting started in single cell research can be daunting. There are a high upfront cost even before running your first samples, the difficulties of generating single cell data, and also the overwhelming vastness of data to analyze. Single cell data are classified as “big data”, which means you need strong computers, and at times, computer servers, to fully analyze data from start to finish.

We understand the struggles of biologists who are getting started in single cell research and we are here to help. Our bioinformatics service, in combination with our RNAdia kit for single cell and single nuclei research, is a great way to get the cellular insight you need without having to learn how to code.

In this webinar, you will learn how we do single cell data analysis at Do Bio, why using our bioinformatics service can help you get started, and how our bioinformatics service fits in with other products and services at Do Bio.

What You Need to Know About Single Cell Sequencing Data Analysis Ask the Experts Session 1

Single cell sequencing gives unrepresented insight into functions of individual cells in health and disease, but this method produces vast amounts of data, as it measures whole-genome expression of thousands of single cells within a single sample. Yet, bioinformaticians are scarce, making data analysis of single cell sequencing data an important (and much sought-after) skill.

With the wealth of analysis options for single cell data out there, it can be daunting for new users. Luckily, there have been a range of tools developed that can help researchers that are new to the data analysis get to grips with their single cell data.

Want to know more about single cell sequencing data analysis? Do you have any burning questions on the tools available and what one is right for your research?

Or do you not have bioinformatic capabilities in- house and want to see if using a Bioinformatic Service like Dolomite Bio’s would be right for you instead?

Watch this webinar recording to discover whether your questions are answered during the course of the presentation and find out where there are further resources to answer your questions.

Getting started: Why should I use a Single Cell Consultancy Service?

Getting started in single cell research can be daunting. Not only is there a high upfront cost to consider before even running your first samples, but there is also the uncertainty of whether the system will be a good fit for your sample type or research question. Alternatively, you might just be looking into running a pilot study or a sample to complement a research paper. In any case, the Single Cell Consultancy Service might be a good way to get started, without the need for capital cost investment.

In this webinar you will learn which research questions are answered by single cell sequencing and why using a single cell service can help you get started.

We will be covering:

• Getting started with single cell sequencing
• Research questions addressed with single cell experiments
• Introduction to our Single Cell Consultancy Service
• How to deliver a single cell project in as little as 2 weeks
• Sample types compatible with our service
• How to cherry-pick the service and support needed to work within your budget and research needs

Introducing the Nadia Innovate – the open platform for single cell research

In recent years, new single cell technologies have revolutionized our understanding of cell identity and diversity and enabled research to be carried out at an unprecedented resolution. The shift from bulk to single cell methods has paved the way for new and exciting research to reveal novel insights in a wide spectrum of biological fields. Take your single cell research to the next level

As researchers uncover the seemingly endless possibilities of single cell research, scientists are looking to increasingly customize existing protocols or develop new protocols and applications to fit with their specific experimental needs.

In this webinar you will discover what single cell research is and what it can do for you. Our speakers will introduce you to Do Bio, our instruments, applications and the Nadia platform workflow, from cells to insights.

Learn how the key benefits and advantages of our platform can help pave the way for new and exciting research, revealing novel insights in a spectrum of applications, which will quickly become the greatest asset in your lab.

The topics include:
– Introduction to Do Bio
– Introduction to single cell research
– Overview of the Nadia Platform
– Single cell application and research areas
– Key benefits of the Nadia Platform.

Optimize Your Single Cell Research – Best Techniques for Sample Preparation

Single cell transcriptomics enables access to the transcriptomes of tissues at single-cell resolution. This powerful tool offers vital information about tissue heterogeneity and cell-specific dynamics that is key to understanding disease, immunity, development and more.

Careful sample preparation is crucial for success in any single cell sequencing experiment. There are several aspects to consider from sample preservation and tissue dissociation to encapsulation of cells or nuclei.
How to address the challenges of single cell research preparation

In this educational webinar, experts from S2 Genomics, Dolomite Bio and Illumina give insights into sample preparation techniques and best practices for scRNA-Seq, sNuc-Seq, ATAC-Seq, and other assays.

The topics include:
– The biggest challenges and top tips for cell and nuclei preparations for single-cell sequencing
– The when and why for using cells vs. nuclei as the proxy for single-cell studies
– Optimizing your sample preparation for single-cell and single-nuclei RNA-seq
– Optimizing your single-cell experiment for different tissues or sample preservation methods
– The preservation of samples for single-cell experiments
– The cell number and Illumina sequencing read depth needed for reliable single-cell analysis

What you always wanted to know about single cell sequencing- Dolomite Bio & Illumina Live Webinar

Single cell sequencing gives unpresented insight into functions of individual cells in health and disease. Although single cell sequencing is applicable for many biological systems, it lacks adoption in many fields. This is due to limiting factors such as cell size incompatibility, poly-A capture chemistry, or lack of customization options on high-throughput commercial single-cell isolation systems.

Do you want to know if high-throughput, single-cell RNA sequencing can be applied to your sample type and if it would be a useful tool to advance your research by shedding new light on biology? Do you have questions about your single cell project that remain unanswered?

If so, this webinar is for you!

Single-cell sequencing experts from Dolomite Bio, the creators of the Nadia platform for single-cell research without constraints, and Illumina, manufacturers of innovative sequencing technologies have created this webinar to answer your burning research questions.

网络讲座 – 单细胞研究的无限探索

近年来，新的单细胞研究技术彻底革新了我们对于细胞鉴定及细胞多样性的理解，使得我们能够以前所未有的分辨率对单细胞进行研究。这种从一群细胞到单细胞研究方法的转变，为我们在生物学领域进行创新的、令人振奋的研究工作铺平了道路，同时提供了新的视角。

这场网络讲座由Mei Wu博士（Dolomite Bio）和APG Bio共同主持，讲座将对Do Bio平台做系统介绍，同时介绍从单细胞制备到获得数据整个实验流程。

通过该讲座学习，您可以获悉Dolomite Bio的Nadia Platform是如何帮助科研工作者在生物学各种应用中进行创新与令人振奋的研究工作，同时提供新的研究视角。

单细胞研究，您触手可及！

演说者: Mei Wu博士, 现任Do Bio高级研发科学家。Mei Wu博士在爱丁堡大学获得化学生物学博士学位，在发展生物高通量筛选技术和分子生物学方向有很多研究经验。 吴博士在领导建立产品研发的同时，也经常参加各类学术会议。

Dolomite Bio & Illumina Live Webinar Analyse your single cell RNA Seq data

Getting started with single-cell RNA-Seq (scRNA-Seq) data analysis and looking at how to implement an easy and reproducible workflow? Have a look at our Illumina & Dolomite Bio webinar on single cell data analysis with our guest speaker Dr. Sebastian Müller (Postdoctoral Research Assistant, University of Cambridge & Founder, Alva Genomics).

Single cell data analysis has become vital to understand gene expression on a single cell level and to identify expression patterns in transcriptomic studies.

Join us in exploring a Nadia dataset using an easy and reproducible workflow. Take control of data quality and understand how to implement crucial quality control steps in your pipeline. Find out how to generate varied graphical outputs quickly to suit your research question.

During this webinar, Dr. Thomas Ayers will also provide an overview of the flexible single-cell analysis solutions offered by Dolomite Bio, and Dr. Pawel Zajac will briefly highlight the latest Illumina sequencing news, enabling deeper and broader single-cell sequencing studies.

Single-cell insights are within your reach!

Dolomite Bio & S2 Genomics Integrated Workflow – Single cell data from mouse lung cells

Flexible End to End Single Cell Workflows- Dolomite Bio & Illumina Live Webinar

The study of single cells has revolutionized our understanding of cell identity and diversity and enabled research to be carried out at an unprecedented resolution. The shift from bulk to single cell methods has paved the way for new and exciting research to reveal novel insights in a spectrum of applications.

In this webinar we discuss the customisable single cell sequencing workflows offered by Do Bio and Illumina to provide an end-to-end solution for varying cell types, sizes and applications.

Dr. Thomas Ayers (Technical Applications Specialist) from Do Bio will cover:
– The Nadia Instrument for scRNA-seq and sNuc-seq.
– The Nadia Innovate for novel protocol development.
– A full workflow overview from cells to data.

Pawel Zajac (Sr. Segment Marketing Manager EMEA, Cell Biology) will describe:
– How Illumina sequencing solutions are an integral part of a wide range of single-cell sequencing applications.
– How the NextSeqTM 2000 Sequencing System allows you to study more cells, additional modalities and expand your research.

Watch the webinar to see how customisable single-cell workflows are within your reach!

Identifying novel full-length mRNAs using single cell isoform sequencing

This webinar, featuring Jason Underwood, Principal Scientist at PacBio, will discuss Do Bio’s Nadia Instrument together with the PacBio Sequel System for single cell isoform sequencing (Iso-Seq™).
Join us to discuss the following topics:

An introduction of long read sequencing to the world of single cell isoform sequencing

Isoform sequencing: more than just a transcript

Using short read 3’ counts and isoform information together

Investigating isoforms with Do Bio’s Nadia Instrument and the PacBio® Sequel® II System

Long reads in action: cerebral organoid single cell Iso-Seq

Single cell analysis with dropSeqPipe

Watch our webinar with Sebastian Muller, a dropSeqPipe development contributor and bioinformatics expert, to find out more about the topics below!

Introduction to dropSeqPipe (dSP) – Learn the basics of setting up dropSeqPipe for your datasets
dropSeqPipe in action – Join us in exploring a Nadia dataset using this easy and reproducible workflow
Taking control of data quality – Understand how to implement crucial Quality Control steps in your pipelines
Tailored visualization – Find out how to generate varied graphical outputs quickly to suit your research question

Customizable single cell protocol and application development

With the option to customize protocols and reagents on the Nadia Innovate, scientists are able to tailor their research to an unlimited range of projects, applications and cell types.

Watch the latest webinar today to:
• Find out how you can extensively customize a range of user parameters to meet the requirements of your single cell experiments
• Learn how to seamlessly optimize protocols and develop new applications with the Nadia Innovate
• Hear a protocol development case study on encapsulating single cells in agarose droplets using the Nadia Innovate
• Get answers to all your single cell research questions in our live Q&A