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scRNA-Seq/sNuc-Seq technical update – Emulsion breakage step

scRNA-Seq/sNuc-Seq technical update – Emulsion breakage step

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Protocol improvement details

Website Small Portrait Image - Nadia LogoBoth scRNA-Seq and sNuc-Seq protocols on the Nadia Instrument are well-established protocols resulting in outstanding gene capture rates for a large variety of different cell types. To further improve and streamline the protocols, Dolomite Bio has replaced the centrifugation-based emulsion break procedure with a filter-based approach.  
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The implementation of this optimized and easy-to-use step within the protocols results in reduced hands-on-time with increased, reproducible bead recovery, whilst retaining exceptional data quality.button

 scRNA-Seq/sNuc-Seq protocol update

The scRNA-Seq and sNuc-Seq protocols have been updated in the highlighted area below. 

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Figure 1: The scRNA-Seq and sNuc-Seq protocols have been updated to include the filter-based emulsion breakage step as a replacement for the centrifuge-based method. 

 

 Workflow benefits 

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–       Reduced hands-on time 
– Decreased number of handling steps
– Decreased number of handling steps
–       Streamlined bead recovery 
– Reduced risk of sample contamination and RNA-degradation
– Reduced risk of sample contamination and RNA-degradation
–       Exceptional data quality

 

Download the updated scRNA-Seq and sNuc-Seq protocols by filling in the form below