Both scRNA-Seq and sNuc-Seq protocols on the Nadia Instrument are well-established protocols resulting in outstanding gene capture rates for a large variety of different cell types. To further improve and streamline the protocols, Dolomite Bio has replaced the centrifugation-based emulsion break procedure with a filter-based approach.
The implementation of this optimized and easy-to-use step within the protocols results in reduced hands-on-time with increased, reproducible bead recovery, whilst retaining exceptional data quality.
The scRNA-Seq and sNuc-Seq protocols have been updated in the highlighted area below.
Figure 1: The scRNA-Seq and sNuc-Seq protocols have been updated to include the filter-based emulsion breakage step as a replacement for the centrifuge-based method.