FAQ

In most cases, yes, your cells will work with the system. Whether a cell is compatible or not is dependent on many things such as the size and fragility of the cells. Please contact our applications team at applications@dolomite-bio.com for a detailed list of cells that have been trialed in our Nadia product family.

Cells up to 60 µm in diameter can be used on the Nadia Instrument. We advise that cells do not exceed this size to avoid blockages.

Yes. The Nadia Instrument supports volumes as small as 100µl.

Cells and beads are advised to be used at a concentration of 300 and 600 per µl, respectively. Lower concentrations can be used, but be aware that this will affect capture rate. 

Yes, we sell the RNAdia kits for single cell RNA-Sequencing containing all reagents from sample encapsulation to sequencing library generation. Find our kits here

• Mouse hybridoma
• CHO
• Human and Mouse B-cells
• Human T-cells
• Cardiomyocytes
• Hepatocytes
• Jurkat cells
• HEK
• 3T3
• Endometrium
• Yeast
• Plant Protoplasts
• Bacterial cells such as E.coli and Streptomyces
• Nuclei
• Genomic DNA
• and many more

To enquire whether you can use the Nadia system with your cell types, contact our applications team at applications@dolomite-bio.com

Typically, 20% larger or smaller than the junction size (this is achieved by varying the pressure/flow rate). On the Nadia instrument using the Innovate module, you can generate droplets between ~65µm and 100µm in diameter.

Around a week at room temperature or two weeks at 4°C when kept in a sealed container to avoid evaporation.

The Dolomite Bio systems provide a solution for encapsulating single cells into droplets with/without beads. The Nadia Instrument generates a set of beads with mRNA attached, ready for downstream processing. The RNAdia kit  then provides everything you need from sample encapsulation to sequencing library generation. If you have queries about the protocol downstream of the encapsulation process, please contact support@dolomite-bio.com.

Yes, the Nadia Instrument when combined with Nadia Innovate is fully flexible allowing the use of own reagents and the development of applications.

All cDNA libraries prepared from the Nadia Instrument are fully compatible with short-read sequencing on Illumina and long-read sequencing on PacBio or Oxford Nanopore sequencing devices.

4000

50 ms

This is dependent on the concentration of the beads you are using, but when using our advised concentrations, the capture rate for cells is 10%. Meaning that 10% of the input cells will be captured, e.g. if you encapsulate 75,000 cells 7,500 will be captured for sequencing.

250µl bead solution, 250µl cell solution and 3ml oil (for 20 min run and highest recommended flow rates).

You can run either 1, 2, 4 or up to 8 samples in parallel in a 20 min run on the Nadia Instrument.

Run parameters such as pressures, stirrer speeds and temperatures can be altered when combining the Nadia Instrument with Nadia Innovate. This enables the Nadia Instrument to perform novel applications or use different buffer compositions for scRNA-seq.

Up to 48,000 single cell libraries in 20 minutes when using an 8-way cartridge on the Nadia instrument.

If you are looking to see the products live and running, we regularly run workshops or demonstrations in many countries around the world. Please get in touch with one of our single cell experts to learn how and when here.

Please visit our webinar page to find out more.

Yes, installation and training can be (and typically for all system purchases, will be) included in the quotation. Please check your quotation.

Installation and training involves a specialist from the Dolomite Bio team visiting your institute to firstly install (set up and test performance of) the system. It also includes dedicated training of you and your colleagues, where we can help you understand the system, how to set it up and give guidance on your application. This often proves essential for users who have not used a microfluidic platform or are unfamiliar with the application and its set up.

If you already have a quote, please email your purchase order to orders@blacktrace.com (Dolomite Bio is a brand of Blacktrace). If you would like a quote, please visit Request a Quote.

If you run into any problems with the system, please contact our support team at support@dolomite-bio.com

The latest version of FCC is downloadable from our download page under software downloads.

Nadia instrument and Nadia Innovate chips are single-use consumables that are discarded after a run.

The Nadia product family does not have wetted parts and therefore doesn't require cleaning. Chips are one time use and are therefore disposed of after every experiment.

We are able to advise you and produce system quotes on products if you are unsure of what you need to run your application. Please contact us.

Dolomite provides microfluidic components, such as pumps, chips and other accessories, which can be used for various different applications or function as microfluidic system solutions.

Dolomite Bio is focused on developing and selling products, typically complete systems and consumables to biologists. Many of these products are focussed on specific biological technologies such as, but not limited to, Single Cell RNA Seq. Some of these products contain microfluidics but this is not the focus of Dolomite Bio.

Yes, vacancies are updated regularly on our Careers page