Single Nuclei RNA-Seq application available on Nadia
Single nuclei RNA-Seq allows researchers to profile gene expression in difficult to isolate cells as well as archived tissue. To enable this application, Dolomite Bio has established high throughput sNuc-Seq on the Nadia Instrument.
As part of this newly established high throughput sNuc-Seq protocol on the Nadia Instrument, Dolomite Bio will provide a tailored protocol for nuclei preparation based on previously published studies (Habib et al. 2017.).
Following nuclei preparation, thousands of single nuclei from fixed or frozen tissue samples are encapsulated with single barcoded mRNA capture beads using droplet microfluidics on the Nadia Instrument. The Nuclei are lysed inside the droplets and their mRNA content is captured in the beads.
Generating reproducible and cost-effective single nuclei data with Nadia
Using the Nadia Instrument, scientists can profile thousands of single nuclei consistently and reproducibly and also allow scientists to capture high numbers of genes with low doublet rates and low cost.
 Habib et al. “Massively parallel single-nucleus RNA-seq with DroNc-seq.” Nature methods 14.10 (2017): 955.