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RNAdia 2.0 kit

RNAdia 2.0 - high quality, low cost, complete solution

 

One solution to take you from cells to data.

With the RNAdia 2.0 kit for the Nadia Instrument, gain access to a hassle free, streamlined workflow, where you can create single cell libraries and analyse them, all in one place.

Single cell research doesn’t need to be a hassle.

Alongside RNAdia 2.0 we now offer our free Droplet Analyzer Software and the Bioinformatics service add on, saving you valuable time spent finding steps of the single cell workflow from other sources.

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RNAdia 2.0 key features

 Cost RNAdia Low cost reagents extremely cost effective when  compared to other products in the field High throughput RNAdia High-throughput capture tens of thousands cells in one run
Throughput flexibility RNAdia Throughput flexibility subsampling enables analysis of just a few hundred cells per sample  Bioinformatics RNAdia No Bioinformatics knowledge required we take care of your data analysis for you- choose the Bioinformatics service add on
streamlined workflow RNAdia Streamlined workflow go from sample encapsulation to amplified cDNA libraries in as little as 6h, allowing you to concentrate on cell preparation if necessary Single Cell RNadia Transcriptomic insight for single cells
Complete workflow RNAdia End-to-end workflow with our RNAdia 2.0 kit and Bioinformatics service you have everything you need to go from cells to data Sample compatibility RNAdia Very high sample compatibility through sample buffer flexibility and broad cell size range

 
 

Our platform
The Nadia Instrument

The Bioinformatics
Service

Droplet Analyzer
software

 

The Nadia Instrument is an automated, microfluidic droplet-based platform used for single cell research that encapsulates up to 8 samples, in parallel, in under 30 mins. Tens of thousands of cells can be captured per cartridge in a run.

The fully automated Nadia Instrument guides users through all relevant steps of the experiment via an easy-to-use touchscreen interface.

The Nadia platform is fully compatible with the RNAdia 2.0 kit for single cell transcriptome analysis.

Learn more >

 

Alongside our RNAdia 2.0 kits you can purchase vouchers for our Bioinformatics service, entitling you to data analysis services as part of a bundle with the RNAdia 2.0 kit!

Voucher codes match the size of the kit, so an 8-sample kit entitles you to 8 vouchers, meaning that you will get 8 samples analyzed.

What’s included?

  • Pre-processing: quality control, filtering, count matrix
  • Preliminary clustering
  • Report: MultiQC summary, Seurat object, UMAP and/or t-SNE, and comments on your data

Detect and analyse hydrogel scaffolds and aqueous droplets generated with microfluidics technology.

Free, easy to use software designed to help researchers accurately calculate droplet
size, bead capture frequency or multiplet proportion with ease.

 

Learn more >

 

 

 

 

A complete workflow

workflow steps RNAdia

Sample preparation

The Nadia Instrument can encapsulate cells of over 50 μm due to its wide chip junction size. This includes cells that require different buffers such as cardiomyocytes and plant cells. Most instruments in the field can only process cells up to 30 µm and use standard sample buffers such as PBS.
Ease of use combined with accommodation of a wide range of cells and sizes.

 

 

Run RNAdia

Single cell library construction on Nadia

The RNAdia 2.0 kit enables the construction of single cell libraries on the Nadia Instrument, which consist of cellular transcripts, ready for sequencing. The Nadia Instrument is an automated, microfluidic droplet-based platform used for single cell research that encapsulates up to 8 samples, in parallel, in under 30 mins.
We now offer droplet visualization using our brand-new Droplet Analyzer Software.

 

Next Generation Sequencing

The pooled barcoded cDNA libraries using the RNAdia 2.0 kit are compatible with standard NGS short-read sequencing using our standard protocol, allowing the analysis of thousands of transcriptional profiles of single cells in parallel. Additionally, due to the presence of full length 3’ cDNA, the protocol is easily amenable to long-read sequencing on Pacific Biosciences or Oxford Nanopore sequencers.
Library sequencing using short or long read sequencing technologies.

Bioinformatics analysis and visualization

Single cell transcriptomic data can be analysed using open-source pipelines or with our in-house service for data analysis. Purchasing our Bioinformatics service add-on is as easy as purchasing a kit.
We offer a complete service for Bioinformatics analysis using our in-house service.

Publish

 

Why trust us?

  • We offer world-class support
  • We have >15 years of experience in droplet microfluidics
  • Our technology has been to the moon and the deep sea
  • With high compatibility and low cost we make single cell research accessible

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Resources

  App Note CTA
  Download the RNAdia 2.0 Application Note Download the datasheet Watch the intro to RNAdia 2.0 video

Q&A

Which reagents for the single cell sequencing workflow does the RNAdia 2.0 kit contain?
The RNAdia 2.0 kit contains reagents for all steps from sample encapsulation, cDNA library preparation, PCR amplification to NGS-library preparation.

When will the RNAdia 2.0 kit be available for order?
RNAdia 2.0 kits will be available for order from the 30th November.

How many samples can be processed with one RNAdia 2.0 kit?
We offer two versions of the RNAdia 2.0 kit, to process either 2 or 8 samples.

Is there a limit to cell size on the Nadia Instrument?
The Nadia Instrument can process cells of over 50µm and those with different buffer requirements such as cardiomyocytes and plant cells which are typically much larger than 50µm in diameter.

How do I analyze my single cell data?
Single cell transcriptomic data can be analyzed using open-source pipelines or with our in-house service for data analysis. You are able to purchase data analysis and visualization from Dolomite Bio as you can a kit.

What sample types are compatible with the Nadia Instrument?
The Nadia Instrument and its associated RNAdia 2.0 kit are compatible with a very wide range of samples types owing to its flexibility in sample buffers and broad cell size range. Species that have been tested internally and published range from mammals, fungi and plants and are sourced from primary tissue and model organisms.

How do I get in touch with Dolomite Bio about the Bioinformatics Service?
Please contact us with your question here and one of our Single Cell Specialist will be in touch with you shortly.

Which kind of samples can be submitted for the Bioinformatics Service?
We accept data generated on the Nadia Instrument from human, mouse, or fruit fly samples.

 

Discover RNAdia 2.0

 

 

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