Long-read sequencing in single cell research promises to revolutionize our understanding of biology and human disease and could have a significant impact on disease diagnostic. Techniques such as offered by PacBio and Oxford Nanopore have the potential to transform the field of full-length transcript sequencing. In single cell transcriptomics, long read sequencing enables to study many cellular mechanisms including splicing events, fusion transcripts, and single nucleotide polymorphisms.
Single cell methodologies to investigate isoform diversity, while still being able to capture the gene expression, are critical to fully comprehend the intricacies of the immune system. Such methods can be used to improve our understanding of infectious disease, autoimmune disorders, and aid vaccine as well as immunotherapy development.
Read how a group at the MDC Berlin used the Nadia Instrument to for single cell RNA sequencing of SARS-CoV-2 infected human cell lines
Using long read sequencing to reliably detect specific fusion transcripts could improve Cancer diagnostic by providing valuable biomarkers. The scRNA-Seq application on Nadia produces full length cDNA libraries that are fully compatible with long read sequencing technologies.
Philpott and colleagues developed a method, called scBUC-Seq, to accurately capture the information from the barcode and UMI regions when using Oxford Nanopore long read sequencing. Their technology permits accurate long-read sequencing of single cell libraries generated using the Nadia platform (Philpott et al. 2021).
The development of innovative methods for long-read sequencing requires the use of new reagents and a workflow that is easily adjustable to new experimental needs. The Nadia and Nadia Innovate offer unprecedented flexibility and low experimental cost allowing for seamless generation of long-read sequencing libraries for PacBio from standard scRNA-Seq libraries generated on Nadia.