The heart, one of the major organs in the body, is exposed to various types of stress throughout life. This can be mechanical stress due to constant contraction, or stress at the molecular level due to the abundance of mitochondria in cardiomyocytes (Yamada and Nomura, 2020). Such stresses can affect the complex arrangement of specialized cardiomyocytes and non-cardiomyocytes, and their dynamic interplay, leading to pathologies; this is the focus for scRNA-Seq in Cardiovascular research.
Over the past five years, single-cell RNA sequencing (scRNA-Seq) has brought new insight into the field of cardiovascular research. By enabling scientists to look at cells independently from each other, scRNA-Seq has led to the identification of rare subpopulations of heart cells, with a possible role in pathogenesis.
A significant hurdle for scRNA-Seq on heart cells is the sheer size of adult mammalian cardiomyocytes (~10 x 100 µm), which makes them challenging to process on high-throughput droplet-based systems (Ackers-Johnson et al, 2018).
The Nadia platform enables the encapsulation of whole adult cardiomyocytes, allowing researchers for the first time to perform scRNA-Seq of heart cells at high throughput and true complexity. Furthermore, the flexibility afforded by the Nadia Innovate enables scientists to fine-tune droplet size as well as buffer composition to best suit their particular samples and the range of cell sizes and cell types they contain.