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µEncapsulator 1 – 2 Reagent Droplet Chip – Hydrophilic

The µEncapsulator 1 – 2 Reagent Droplet Chip 15µm hydrophilic chip enables in vitro evolution, by forming millions of 2 picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution.

Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 1 – 2 Reagent 15µm fluorophilic chip (3200527), to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator 15µm hydrophilic chip (3200528). The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.
   

  • Rapid generation of 2pl double emulsion droplets (an aqueous core, surrounded by an oil shell)
  • Channel cross–section at the junction 15µm x 20µm (depth x width)
  • Channel cross–section after the junction 15µm x 115µm (depth x width)
  • Chip size 11.25mm x 15mm x 2mm
  • Attached FKM 0.5mm thick gasket for chip–to–chip connection
  • Integrated flow resistors for excellent flow stability
  • On–chip filter on droplet lines with pore size 15µm x 20µm (depth x width)
  • On–chip filter on carrier oil line with pore size 7.5µm x 20µm (depth x width)
  • 100µm scale marks at the junction for imaging
  • Fabricated in glass
  • Very smooth channels (surface roughness Ra of 5nm)
  • Excellent optical clarity
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