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Dolomite Bio updates single cell RNA-Seq and single nuclei RNA-Seq protocols to reduce hands-on time, increase bead recovery and offer exceptional data quality

Dolomite Bio, a leading provider of high throughput single-cell technology, today announced an update to their single cell RNA-Seq (scRNA-Seq) and single nuclei RNA-Seq (sNuc-Seq) protocols. scRNA-Seq and sNuc-Seq protocols on the Nadia Instrument are well-established protocols resulting in outstanding gene capture rates for a large variety of different cell types. The protocols have been improved to replace the centrifugation-based emulsion break procedure with a filter-based approach, which significantly reduces hands on time, increases bead recovery and retains exceptional data quality.

scRNA-Seq/sNuc-Seq protocol update

Emulsion breakage graphic

Figure 1: The scRNA-Seq and sNuc-Seq protocols have been updated to include the filter-based emulsion breakage step as a replacement for the centrifuge-based method. 

The updated protocol enables:

  • Reduced hands on time (<10 minutes) – Decreased number of handling steps and reduced risk of sample contamination and RNA-degradation
  • Streamlined bead recovery – Improved bead recovery with enhanced reproducibility
  • Exceptional data quality – Comparable gene capture compared to manual methods plus consistent data quality

For more information, please download the protocols here.