New protocols to reduce hands-on time, increase bead recovery and offer exceptional data quality

Dolomite Bio, a leading provider of high throughput single-cell technology, today announced an update to their single cell RNA-Seq (scRNA-Seq) and single nuclei RNA-Seq (sNuc-Seq) protocols. scRNA-Seq and sNuc-Seq protocols on the Nadia Instrument are well-established protocols resulting in outstanding gene capture rates for a large variety of different cell types. The protocols have been improved to replace the centrifugation-based emulsion break procedure with a filter-based approach, which significantly reduces hands on time, increases bead recovery and retains exceptional data quality.

Single nuclei RNA-Seq and single cell RNA-Seq protocol update

Emulsion breakage graphic

Figure 1: The scRNA-Seq and sNuc-Seq protocols have been updated to include the filter-based emulsion breakage step as a replacement for the centrifuge-based method. 

The updated protocol enables:

  • Reduced hands on time (<10 minutes) – Decreased number of handling steps and reduced risk of sample contamination and RNA-degradation
  • Streamlined bead recovery – Improved bead recovery with enhanced reproducibility
  • Exceptional data quality – Comparable gene capture compared to manual methods plus consistent data quality

To find out more information, visit our downloads page to access datasets, application notes, flyers and brochures, which will provide further information on these applications