
In what form is the hydrogel supplied in the nadia3D kit?
The hydrogel in the nadia3D kit is provided as a liquid which must be kept cold during processing.
How many samples can I run in parallel?
You can run from 1 to 8 samples in parallel.
How many cells can you run?
Up to 5000 cells/ul.
How do you recover cells from scaffolds?
The use of a combination of collagenase, DMEM and CaCl2 can help dissolve scaffolds while maintaining cell viability. See application note for more details. Note: the reagents for digesting collagen scaffolds are not provided in the nadia3D kit
How long can you store scaffolds after recovery?
The collagen scaffolds can remain stable for up to a week; however, we recommend immediately processing the scaffolds due to cell viability.
What is the size of scaffolds produced?
Median size is 80 um