Is the agarose provided in the kits in powder form?
The agarose aliquots are provided as a solid gel that is melted before use.
How many samples can I run in parallel?
You can run from 1 to 8 samples in parallel.
How many cells can you run?
Up to 5000 cells/ul.
How can you recover cells from the agarose scaffolds?
The use of a combination of β-agarase, β-agarase buffer, and DMEM can help dissolve scaffolds while maintaining cell viability. See application note for more details. Note: the reagents for digesting agarose scaffolds are not provided in the nadAROSE kit.
How long can you store scaffolds after recovery?
The agarose scaffolds can remain stable for up to a week; however, we recommend immediately processing the scaffolds due to cell viability.
What is the size of scaffolds produced?
Median size is 75 um.
Is the nadAROSE protocol suitable for 3D cell culture?
Agarose is not a suitable substrate for promoting mammalian cell growth, so it is not suitable for 3D cell culture. For a 3D cell culture kit, please refer to the nadia3D kit.