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nadAROSE kit

nadAROSE kit

 

Construct the future of single cell research with the nadAROSE kit

A simple, low cost, commercial solution for the encapsulation of up to a million single cells in individual micro-scaffolds in a high throughput, reproducible manner.

 

Why use agarose for your single cell projects?

  Agarose’s spherical scaffold structure and ability to allow for the diffusion of nutrients and gases make it an ideal system for naturalistic mammalian and non-mammalian live cell studies, given its many similarities to the native physiological environment of living cells. Single cells encapsulated in agarose can, therefore, be maintained for extended periods of time within these individual three-dimensional microenvironments and used in various research including single cell experiments.

nadAROSE downstream applications

Control over monoclonality for your downstream workflows, with applications in:

  • Cell secretion studies
  • Cell-cell interactions
  • Microbial cell culture
  • Compatible with cytometric analyses

Features and benefits of nadAROSE

High throughput encapsulation of up to 1 million cell in one run No microfluidic knowledge required – all scaffold production done with single use, prefabricated consumables
Throughput flexibility RNAdia Throughput flexibility choose to run 1, 2, 4 or 8 lanes at once Sample compatibility RNAdia Very high sample compatibility – broad cell size range
streamlined workflow RNAdia Compatible with cytometric analyses –  cell-containing agarose scaffolds can be flow-processed for cell analysis Experimental flexibility – cell-containing agarose scaffolds are suitable for a range of experimental procedures
Temperature controlled process for robust encapsulation and scaffold construction Compatible with cell release
The nadAROSE kit produces agarose scaffolds that can be later digested to release cells

 

 

Our platform

 

The Nadia Instrument

The Nadia Instrument is an automated, microfluidic droplet-based platform for single cell research that encapsulates up to 8 samples, in parallel, in under 20 mins. Up to a million single cells can be captured per cartridge in a run.

The fully automated Nadia Instrument guides users through all relevant steps of the experiment via an easy-to-use touchscreen interface. With single use chips and flexible control over parameters such as temperature and pressure, the Nadia instrument is ideally suited for your agarose research projects.

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A complete workflow

Sample encapsulation RNAdia

Sample preparation

Nadia can process cells of various sizes, including over 50 um in length such as cardiomyocytes. The nadAROSE kit is designed to be used with PBS buffer.

Wide sample compatibility: Nadia can process cells of various sizes, including large cells over 50 um.

 

Run RNAdia

 

Single cell scaffold production on Nadia

Single cell-containing agarose scaffolds are constructed on Nadia using the nadAROSE kit. The Nadia is an automated, microfluidic droplet-based platform for single cell research that encapsulates up to 8 samples, in parallel, in under 20 mins. Up to one million single cells can be captured in a single run.

Standardised, reproducible encapsulation of cells in agarose.

 

Experimentation

Experiment with your cell-containing agarose scaffolds! What you do now totally depends on what you want to find out! From secretomes to cell-cell interactions, to bacterial culture or cytometric analyses.

Single cell-containing agarose scaffolds are compatible with a variety of downstream workflows.

 

 

 

Cell release

The scaffolds produced with the nadAROSE kit can be later digested to release the cells captured inside them. If you want to subject them to a set of entirely different conditions or process them for particular analyses, you can.

Cells can be liberated from their scaffolds for further analyses.

 

Publish

 

Learn more

 
Register for the webinar:
Hydrogels, Microfluidics, and Cell Biology
Download the datasheet  

 

FAQS

Is the agarose provided in the kits in powder form?
The agarose aliquots are provided as a solid gel that is melted before use.

How many samples can I run in parallel?
You can run from 1 to 8 samples in parallel.

How many cells can you run?
Up to 5000 cells/ul.

How can you recover cells from the agarose scaffolds?
The use of a combination of β-agarase, β-agarase buffer, and DMEM can help dissolve scaffolds while maintaining cell viability. See application note for more details. Note: the reagents for digesting agarose scaffolds are not provided in the nadAROSE kit.

How long can you store scaffolds after recovery?
The agarose scaffolds can remain stable for up to a week; however, we recommend immediately processing the scaffolds due to cell viability.

What is the size of scaffolds produced?
Median size is 75 um.

Is the nadAROSE protocol suitable for 3D cell culture?
Agarose is not a suitable substrate for promoting mammalian cell growth, so it is not suitable for 3D cell culture. For a 3D cell culture kit, please refer to the nadia3D kit.

 

Take the first step towards building the future of single cell research

 

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