Single nucleus RNA-Seq (sNuc-Seq)
The single nucleus RNA-Seq chip is ideal for performing the DroNc-Seq1 protocol. The modified single cell RNA-Seq (scRNA-Seq) methodology which uses many of the same principles as Drop-seq2, uses isolated nuclei as a proxy for whole cells. Using droplet microfluidics this enables users to profile thousands of nuclei at low cost and at high throughput.
Nuclei can usually be rapidly and easily isolated from lightly fixed and frozen tissues, without the extended incubations and processing required for isolating single cells. The rapid isolation of nuclei from lightly fixed or frozen tissue will enable researchers to acquire transcriptomes that are relatively unperturbed by the cell isolation procedure or from tissues it’s difficult to isolate single cells from 3,4,5. Remarkably, the transcriptional profiles from isolated nuclei are surprisingly similar in coverage to those from whole cells, albeit with a greater representation of nuclear RNA and pre-mRNA.
DroNc-Seq uses smaller droplets than Drop-seq to accommodate the lower amount of RNA in nuclei compared to whole cells. To enable researchers to perform DroNc-seq, Dolomite Bio are releasing a modified scRNA-Seq chip that makes smaller droplets. The single nucleus RNA-Seq chip for DroNc-Seq is compatible with Dolomite Bio’s RNA-Seq system.
The single nucleus RNA-Seq chip for DroNc-Seq is available now. Find out more.
Download the Single Nucleus RNA-Seq Application Note
Enter your details below to gain immediate access to the application note
1Habib, N., et al. “Massively parallel single-nucleus RNA-seq with DroNc-seq.” Nature methods 14.10 (2017): 955.
2Macosko, Evan Z., et al. “Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets.” Cell 161.5 (2015): 1202-1214.
3Habib, Naomi, et al. “Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons.” Science 353.6302 (2016): 925-928.
4Lake, Blue B., et al. “Neuronal subtypes and diversity revealed by single-nucleus RNA sequencing of the human brain.” Science 352.6293 (2016): 1586-1590.
5Krishnaswami, Suguna Rani, et al. “Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons.” Nature protocols 11.3 (2016): 499.