Pumps & flow sensors
Dolomite Bio’s Pressure Pumps are used to pump cell suspensions, bead suspensions and oil into microfluidic chips to
generate droplets. They offer ultra-smooth flow (essential for monodisperse droplets) from 0.07μl/min to 10ml/min at pressures up to 10 bar.
They are biocompatible, accurate and offer automated control of flow rate in combination with Dolomite Bio’s flow sensors.
There are two major types of microfluidic droplet chip used in Dolomite Bio systems: the Single Cell RNA Seq chips and µEncapsulator chips.
Both of these chips enable the mixing of two aqueous phases (e.g. cells and beads) immediately prior to dropletization using flow-focusing method.
Chips are internally coated to offer the most desirable surface properties.
The Single Cell RNA Seq chip is designed specifically for implementing the Drop-Seq protocol whereas the µEncapsulator chips are designed for general droplet biology applications and creation of double emulsions.
Injection Valve and Sample Loop for scRNA-Seq
The Injection Valve and Sample Loop for scRNA-Seq from Dolomite Bio enables easy and gentle
introduction of evenly distributed mRNA capture beads into the scRNA-Seq System. This allows beads to be individually encapsulated into droplets with single cells for scRNA-Seq applications.
Dolomite Bio’s microfluidic chips connect to the “macro world” via Multiflux® connectors and interfaces. These enable fast, leak-free and reliable 4-way inter-connections between chips and tubing (which ultimately leads to the pump). The finger-tight fittings allow all 4 connections to be made simultaneously. They allow the making and breaking of hundreds or perhaps thousands of connections prior to replacing seals.
The TCU-100 temperature controller enables extremely accurate control of chips or the µEncapsulator.
It provides and controls a fixed temperature from 1⁰C to 100⁰C, or generates rapid temperature ramps or thermocycling.
The µEncapsulator module is designed specifically for high throughput single-cell applications. It includes a sample reservoir chip (into which 100µl samples can be easily pipetted), a user changeable droplet chip and a collection reservoir.
The fluidic path is optimised for clean passage of cells or beads to the droplet chip where encapsulation occurs. The microfluidic chips drop into defined locations in the module and reliable connections are easily made by closing the lid. Once assembled, the chips and the integrated collection chamber are thermally insulated and can be temperature controlled using the TCU-100.
The µEncapsulator module is designed for use with the Dolomite High Speed Digital Microscope.
Microscope and analysis software
The High Speed Digital Microscope is designed specifically for microfluidic droplet analysis.
This high resolution imaging system allows users to conveniently monitor high speed microfluidic events such as
droplet formation. It ensures that millimetre to micrometre scale features can be visualised clearly, with the
benefit of an extra-long working distance. This allows clear imaging of moving droplets, beads or cells, enabling
quantitative analysis of droplet size at lower flow rates – typically below 1 kHz – and qualitative evaluation at
production speeds of 2-15 kHz or higher, depending on droplet size.
The Droplet Monitor Software is used for measuring droplet size, frequency, and monodispersity.
The Flow Control Centre (FCC) Advanced software enables accurate and simultaneous control of Dolomite Bio’s
systems and modules via a PC. It integrates control and analysis of pressures, flow rates, temperatures and
visualization of microscope feed. FCC Advanced enables automation via “FCC protocols”, which are automated
“wizard-like” scripts that control the systems.
Specialist droplet chemicals
Pico-Surf™ surfactants are biocompatible specialised chemical solutions containing a proprietary
biocompatible surfactant in a fluorocarbon carrier oil. They are ideal for generation of robust
and stable picodroplets over a wide temperature range.