Single nuclei RNA-Seq (sNuc-Seq) is a novel methodology which uses isolated nuclei instead of whole cells to profile gene expression in cells which are difficult to isolate, as well as archived tissue. Using droplet microfluidic technology this enables users to profile thousands of nuclei at low cost and at high throughput.
A recently published method, ‘DroNc-Seq’ (1) by Habib et al. described the use of single nuclei as a proxy for whole cells to generate thousands of single nuclear transcriptomes. This allows the use of cells that cannot be readily dissociated or are fixed. This method which, has been adapted for use on the Nadia platform, is called single nuclei RNA sequencing (sNuc-Seq).
Like the Drop-seq method, sNuc-seq uses droplet microfluidics to encapsulate single nuclei with uniquely barcoded mRNA capture beads. Following downstream processing and sequencing, the transcriptomes of thousands of single nuclei can be analysed, and unique cell types identified.