Single cell RNA-Seq (scRNA Seq) is a tool that enables simple and robust access to the transcriptomes of thousands of single cells – giving unprecedented insight into tissues at single cell resolution. This offers vital information about heterogeneity and cell-specific dynamics, which is key to understanding many diseases, immunity, development and more.
This is in contrast to traditional techniques, that either allowed analysis of a few genes in thousands of individual cells (e.g., in situ hybridization), or the expression profile of thousands of genes, but only on a tissue homogenate.
ScRNA-Seq with RNAdia on the Nadia instrument is a simple and robust, high throughput, single cell transcriptomics droplet-based protocol based on a single cell RNA-Seq method published in 2015 (Macosko E., et al. “Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets.” Cell 161:1202). Streamlined improvements to the original DropSeq protocol allow thousands of cells to be captured individually in droplets, together with lysis reagents and a barcoded bead, all within a few minutes.
Dolomite Bio’s Nadia Instrument allows users to generate high-quality single cell data with a high gene capture rate, at a low cost per cell thanks to the Nadia platform’s reagent flexibility.
The fully automated Nadia Instrument guides users through all the relevant scRNA-Seq steps to encapsulate up to 8 samples in parallel in under 20 minutes. Over 50,000 single cells can be captured per cartridge in a single run. Once sequenced Nadia data can be seamlessly analyzed by easy to use and affordable bioinformatic pipelines such as dropSeqPipe or Partek Flow.