Single cell RNA-Seq (scRNA Seq) is a tool that enables simple and robust access to the transcriptomes of thousands of single cells – giving unprecedented insight into tissues at the level of individual cells. This offers vital information and data, which is key to understanding many diseases and immunity.
This method enables analysis of millions of single cells in a high-throughput manner. This is in contrast to traditional techniques, that either allowed analysis of a few genes in thousands of individual cells (e.g., in situ hybridization), or the expression profile of thousands of genes, but only on a tissue homogenate.
Understanding tumour heterogeneity and clonal evolution – including studying lineage analysis, cancer stem cells, and drug-resistant and metastatic clones.
Understanding complex tissues (e.g. neural tissues).
High resolution identification of cell types and markers, and understanding differentiation pathways in developmental and systems biology.
To meet the demand for simple and robust, high throughput, single cell transcriptomics, reliable droplet-based protocols for single cell RNA-Seq were published in 2015 (Macosko E., et al. “Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets.” Cell 161:1202). These allow thousands of cells to be captured individually in droplets, together with lysis reagents and a barcoded bead, all within a few minutes.
Dolomite Bio’s Nadia Instrument allows users to perform scRNA-Seq in order to generate high-quality single cell data with a high gene capture rate, at a lower cost.
The fully automated Nadia Instrument guides users through all the relevant Drop-seq steps to encapsulate up to 8 samples, in parallel in under 20 minutes. Over 50,000 single cells can be captured per cartridge in a run. Once sequenced Nadia data can be seamlessly analyzed in Partek Flow.