What is the capture efficiency of cells?

This is dependent on the concentration of the beads you are using, but when using our advised concentrations, the capture rate for cells is 10%. Meaning that 10% of the input cells will be captured, e.g. if you encapsulate 75,000 cells 7,500 will be captured for sequencing.

How much oil/cells/beads do you consume in one standard scRNA-Seq run?

250µl bead solution, 250µl cell solution and 3ml oil (for 20 min run and highest recommended flow rates).

How many samples can I run in parallel?

You can run either 1, 2, 4 or up to 8 samples in parallel in a 20min run on the Nadia Instrument.

Can I change droplet size or buffer compositions on the Nadia Instrument?

Run parameters such as pressures, stirrer speeds and temperatures can be altered when combining the Nadia Instrument with Nadia Innovate. This enables the Nadia Instrument to perform novel applications or use different buffer compositions for scRNA-seq.

How many single cell libraries can you make per hour?

Up to 48,000 single cell libraries in 20 minutes when using an 8-way cartridge on the Nadia instrument.

Single cell RNA-Seq

Single cell RNA-Seq (scRNA Seq) is a tool that enables simple and robust access to the transcriptomes of thousands of single cells – giving unprecedented insight into tissues at single cell resolution. This offers vital information about heterogeneity and cell-specific dynamics, which is key to understanding many diseases, immunity, development and more.

This is in contrast to traditional techniques, that either allowed analysis of a few genes in thousands of individual cells (e.g., in situ hybridization), or the expression profile of thousands of genes, but only on a tissue homogenate.

The Nadia Instrument takes scRNA-Seq to the next level by using automation and flexibility to generate high quality reproducible single cell data at high throughput to assay tens of thousands of cells. Together with RNAdia reagent kit and workflow this becomes a low cost, high quality, complete workflow for single cell sequencing.

RNAdia on the Nadia Instrument enables:

More projects within budget!
Unrivaled versatility with reagents & custom protocols
Exceptional data yield
Unprecedented sample compatibility with diverse cell types

Single cell transcriptome sequencing enables new types of analyses, for instance:

Understanding tumour heterogeneity and clonal evolution – including studying lineage analysis, cancer stem cells, and drug-resistant and metastatic clones.
Understanding complex tissues with diverse cell compositions (e.g. brain, tumour, plant leaf).
High resolution identification of cell types and marker genes, and understanding differentiation pathways in developmental and systems biology.

ScRNA-Seq with RNAdia on the Nadia instrument is a simple and robust, high throughput, single cell transcriptomics droplet-based protocol based on a single cell RNA-Seq method published in 2015 (Macosko E., et al. “Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets.” Cell 161:1202). Streamlined improvements to the original DropSeq protocol allow thousands of cells to be captured individually in droplets, together with lysis reagents and a barcoded bead, all within a few minutes.

Dolomite Bio’s Nadia Instrument allows users to generate high-quality single cell data with a high gene capture rate, at a low cost per cell thanks to the Nadia platform’s reagent flexibility.

The fully automated Nadia Instrument guides users through all the relevant scRNA-Seq steps to encapsulate up to 8 samples in parallel in under 20 minutes. Over 50,000 single cells can be captured per cartridge in a single run. Once sequenced Nadia data can be seamlessly analyzed by easy to use and affordable bioinformatic pipelines such as dropSeqPipe or Partek Flow.

Download our RNAdia on Nadia application note below

Associated products

Nadia Instrument