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Single cell RNA-Seq

Single cell RNA-Seq (scRNA Seq) is a tool that enables simple and robust access to the transcriptomes of thousands of single cells  – giving unprecedented insight into tissues at single cell resolution. This offers vital information about heterogeneity and cell-specific dynamics, which is key to understanding many diseases, immunity, development and more.

This is in contrast to traditional techniques, that either allowed analysis of a few genes in thousands of individual cells (e.g., in situ hybridization), or the expression profile of thousands of genes, but only on a tissue homogenate.

 

The Nadia Instrument takes scRNA-Seq to the next level by using automation and flexibility to generate high quality reproducible single cell data at high throughput to assay tens of thousands of cells.

ScRNA-Seq on the Nadia Instrument enables:

  • More projects within budget!
  • Unrivaled versatility with reagents & custom protocols
  • Exceptional data yield
  • Unprecedented sample compatibility with diverse cell types

Single cell transcriptome sequencing enables new types of analyses, for instance:

  • Understanding tumour heterogeneity and clonal evolution – including studying lineage analysis, cancer stem cells, and drug-resistant and metastatic clones.

  • Understanding complex tissues with diverse cell compositions (e.g. brain, tumour, plant leaf).

  • High resolution identification of cell types and marker genes, and understanding differentiation pathways in developmental and systems biology.

ScRNA-Seq on Nadia is a simple and robust, high throughput, single cell transcriptomics droplet-based protocol based on a single cell RNA-Seq method published in 2015 (Macosko E., et al. “Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets.” Cell 161:1202). Streamlined improvements to the original DropSeq protocol allow thousands of cells to be captured individually in droplets, together with lysis reagents and a barcoded bead, all within a few minutes. 

Dolomite Bio’s Nadia Instrument allows users to generate high-quality single cell data with a high gene capture rate, at a low cost per cell thanks to the Nadia platform’s reagent flexibility. 

The fully automated Nadia Instrument guides users through all the relevant scRNA-Seq steps to encapsulate up to 8 samples in parallel in under 20 minutes. Over 50,000 single cells can be captured per cartridge in a single run. Once sequenced Nadia data can be seamlessly analyzed by easy to use and affordable bioinformatic pipelines such as dropSeqPipe or Partek Flow.

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