Lipid Nanoparticles
Easy screening of LNP’s for early target identification
Introduction
Many researchers have been producing LNPs with expertise and equipment from our sister brand Dolomite Micro. Likewise our other sister brand Particle Works produces instrumentation for LNP screening and scale-up production to the pharmaceutical industry. Do Bio draws on experience from both of these brands to develop the Nadia product family; a powerful platform for producing LNPs without needing microfluidic expertise. The Nadia product family is one of the easiest, fastest and most cost effective microfluidic platforms on which to screen LNP cargo candidates for drugs, vaccines or other therapies.
What are LNPs?
Lipid nanoparticles or LNPs are nanoscale particles used for drug delivery into cells. A cargo, such as mRNA or plasmid DNA, can be delivered into cells by a LNP carrier, altering the cell’s physiology.
Interest in LNPs has exploded since their development into vaccines for Covid 19. LNPs are potentially a critical thereupeutic tool to prevent infectious disease, treat cancer and genetic conditions.
Powering LNP production with microfluidics
Why take a microfluidic approach?
Production of LNPs requires controlled microscale mixing of lipid formulations and nucleic acid cargo, this can only be reliably achieved with microfluidics.
The Nadia platform is a turnkey microfluidic solution developed specifically for life science research and can be easily used by biologists to achieve the microscale mixing required to produce LNPs. Therefore, researchers can screen cargo candidates for LNPs using the Nadia, without the need for proficiency in complex microfluidic set ups.
What makes the Nadia an easy and convenient
solution for LNP production?
Make the next revolutionary therapuetic discovery.
The Nadia platform has many features that make it the ideal instrument to produce LNPs for cargo screening.
Ease of use
Compact, contained microfluidic system with simple pippette loading, touch screen interface and sample loading guide lights
Scalability
Capable of processing 1, 2, 4 or 8 samples in parallel
Temperature control
Easy temperaure control for optimium lipid solubility and cargo stability
Single use chips
Disposable chips with no wetted parts to avoid cross contamination.
Customizable
Open configurable system to control total flow rates and flow rate ratios
Capabilities
LNP’s can be used in the following research areas:
The Nadia platform has several features which make it ideal for LNP and liposome production. Three independent pressure pumps permit manipulation of flow rate ratios and total flow rates while temperature control can guarantee solubilisation of lipids.
The Nadia instrument has been used to produce LNPs and liposomes with industry standard lipids, including SM102, DSPC DMPC and DSPE. mRNA containing LNPs can be produced on the Nadia with sizes ranging from 150-250 nm with a PDIs of 0.15.
Interested in the production of LNPs with the Nadia Instrument? Get in touch!
3 replicates of liposome preparation on the Nadia with a lipid formulation containing DMPC and DSPE.
Vaccines
Cancer therapies
