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Hydrogels are semi-solid materials composed of a network of insoluble hydrophilic polymers which absorb water. Hydrogels can either be naturally occurring, such as agarose, acrylamide, or collagen; or chemically synthesised, such as polyethylene glycol.
The nadAROSE kit provides a simple, low-cost commercial solution for the encapsulation of single cells in individual agarose micro-scaffolds in a high throughput, reproducible manner on the Nadia instrument. Due to the high-throughput the cost per cell is drastically reduced compared to other methods.
The nadAROSE kit is compatible with many downstream applications. The cell-containing agarose scaffolds are compatible with flow cytometry instruments and could also be used for cell-cell interaction studies, cell secretome studies or for microbiological cell culture, to name a few. Whatever the application, you can do it while maintaining single cell identity. The nadAROSE kit also provides the necessary reagents cells from their agarose scaffolds for further cell analysis or merging of workflows.
3D cell cultures, such as organoids and spheroids, have distinct advantages over 2D cultures for diverse cell and tissue types as they more closely mimic the natural environments of the cell. The first step in many 3D cell culture protocols is to seed single or multiple cells into a hydrogel matrix or scaffold.
The nadia3D kit provides a simple, commercial solution for the encapsulation and culture of single-cells in individual collagen-based micro-scaffolds in a high-throughput and reproducible manner using the Nadia instrument.
 Luo R-C. and Chen C-H., “Structured Microgels through Microfluidic Assembly and their Biomedical Applications”, Soft. 2012 Dec;1;1-23
 Smith BH, Gazda LS, Conn BL, Jain K, Asina S, Levine DM, et al. Three-Dimensional Culture of Mouse Renal Carcinoma Cells in Agarose Macrobeads Selects for a Subpopulation of Cells with Cancer Stem Cell or Cancer Progenitor Properties. Cancer Res. 2011 Feb 1;71(3):716–24.
 Li Y, Kumacheva E. Hydrogel microenvironments for cancer spheroid growth and drug screening. Sci Adv. 2018 Apr 1;4(4):eaas8998.
 Joseph JS, Malindisa ST, Ntwasa M. Two-Dimensional (2D) and Three-Dimensional (3D) Cell Culturing in Drug Discovery. In: Cell Culture [Internet]. 2018 [cited 2019 Sep 19]. Available from: https://www.intechopen.com/books/cell-culture/two-dimensional-2d-and-three-dimensional-3d-cell-culturing-in-drug-discovery