Droplet Genomics

High resolution profiling of biological systems powered by microfluidics

Droplet genomics is a technology that allows compartmentalized picolitre reactions to be carried out in a high throughput fashion. scRNA-seq and sNuc-seq are methodologies where isolated cells or nuclei are used to profile gene expression. The Nadia product range can readily encapsulate cells, nuclei and other biologicals using microfluidic technology. Analyze your biologicals at high resolution, singulated by over thousands of rapidly generated droplets.

Single cell RNA sequencing

Single cell RNA Sequencing (scRNA-Seq) is a tool that provides access to the transcriptomes of thousands of single cells – giving unprecedented insight into tissues at single cell resolution. This offers vital information about heterogeneity and cell-specific dynamics, which is key to understanding many diseases, immunity, development and more. This is in contrast to traditional techniques, that either allowed analysis of a few genes in thousands of individual cells (e.g., in situ hybridization), or the expression profile of thousands of genes, but only on homogenized samples.

Single nuclei RNA sequencing

Single nuclei RNA Sequencing (sNuc-Seq) is a technique which uses isolated nuclei instead of whole cells to profile gene expression in cells which are difficult to isolate. Using droplet microfluidic technology this enables users to profile thousands of nuclei at low cost, in a highly parallel method

How it works

SNuc-seq uses droplet microfluidics to encapsulate single nuclei with uniquely barcoded mRNA capture beads. Following downstream processing and sequencing, the transcriptomes of thousands of single nuclei can be analysed, and unique cell types identified.

sNuc-seq uses droplet microfluidics to encapsulate single nuclei with uniquely barcoded mRNA capture beads. Following downstream processing and sequencing, the transcriptomes of thousands of single nuclei can be analysed, and unique cell types identified.

Nuclei can usually be rapidly and easily isolated from frozen tissues, without the extended incubation and processing required for isolating single cells. The rapid isolation of nuclei from complex tissue will allow researchers to acquire transcriptomes that are relatively unperturbed by the isolation procedure.

RNAdia 2.0 reagent kit for cells and nuclei transcriptomics

One solution to take you from cells to data.

With the RNAdia 2.0 kit for the Nadia Instrument, gain access to a hassle free, streamlined workflow, where you can create single cell and single nuclei transcriptome libraries and analyse them, all in one place.

Single cell research doesn’t need to be a hassle.

The Nadia Instrument takes scRNA-Seq and sNuc-Seq to the next level by using automation and flexibility to generate high quality reproducible single cell data at high throughput to assay tens of thousands of cells or nuclei. Together with the RNAdia 2.0 reagent kit and its streamlined workflow, this becomes a low cost, high quality, complete workflow for single cell sequencing and single nuclei sequencing.

Alongside RNAdia 2.0 we now offer our free Droplet Analyzer Software and the Bioinformatics service add on, providing you with all the tools required to answer your research questions.

RNAdia 2.0 key features

Low cost reagents

Extremely cost effective when compared to other products in the field

Streamlined workflow

Go from cell encapsulation to amplified cDNA libraries in as little as 6h, allowing you to concentrate on cell preparation if necessary

End-to-end workflow

With our RNAdia 2.0 kit and Bioinformatics service you have everything you need to go from cells to data

Very high sample compatibility

Our microfluidic chip design is compatible with a broad range of cell sizes



Tumour heterogeneity
and clonal evolution

Understanding complex tissues with diverse cell compositions (e.g. brain, tumour, plant leaf).

Complex tissues with
diverse cell compositions

High resolution identification of cell types and marker genes, and understanding differentiation pathways in developmental and systems biology.

High resolution identification of cell types and marker genes

Products and services for the complete RNAdia 2.0 workflow


Take your research to the next level with Nadia- our microfluidic technology and pre-optimized processes, your analysis and discovery

  • Select microfluidic steps defined by us
  • Deploy up to 8 samples in parallel
  • Measure- analyze your results; answer your research question

Bioinformatics service

Alongside our RNAdia 2.0 kits you can purchase vouchers for our Bioinformatics service, entitling you to data analysis services as part of a bundle. Voucher codes match the size of the kit, so an 8-sample kit entitles you to 8 vouchers, meaning that you will get 8 samples analyzed.

What’s included?

  • Pre-processing: quality control, filtering, count matrix
  • Preliminary clustering
  • Report: MultiQC summary, Seurat object, UMAP and/or t-SNE, and comments on your data

Droplet Analyzer software

Detect and analyze hydrogel scaffolds and aqueous droplets generated with microfluidics technology.Free, easy to use software designed to help researchers accurately calculate droplet size, bead capture frequency or multiplet proportion with ease.

Learn more about RNAdia


RNAdia 2.0 for cells app note


The RNAdia 2.0 intro video


Download RNAdia 2.0 for nuclei application note


Is there a limit to biologicals size on the Nadia Instrument?

The Nadia Instrument can process biologicals of over 50µm and those with different buffer requirements such as cardiomyocytes and plant cells which are typically much larger than 50µm in diameter.

How many samples can I run in parallel?

You can run from 1 to 8 samples in parallel.

How many cells can you run?

Up to 5000 cells/ul.

What sample types are compatible with the Nadia Instrument?

The Nadia Instrument and its associated RNAdia 2.0 kit are compatible with a very wide range of samples types owing to its flexibility in sample buffers and broad cell size range. Species that have been tested internally and published range from mammals, fungi and plants and are sourced from primary tissue and model organisms.

How do I analyze my single cell data?

Single cell transcriptomic data can be analyzed using open-source pipelines or with our in-house service for data analysis. You are able to purchase data analysis and visualization from Do Bio as you can a kit.