Droplet Genomics
High resolution profiling of biological systems powered by microfluidics
Droplet genomics is a technology that allows compartmentalized picolitre reactions to be carried out in a high throughput fashion. scRNA-seq and sNuc-seq are methodologies where isolated cells or nuclei are used to profile gene expression. The Nadia product range can readily encapsulate cells, nuclei and other biologicals using microfluidic technology. Analyze your biologicals at high resolution, singulated by over thousands of rapidly generated droplets.
Single cell RNA sequencing
Single cell RNA Sequencing (scRNA-Seq) is a tool that provides access to the transcriptomes of thousands of single cells – giving unprecedented insight into tissues at single cell resolution. This offers vital information about heterogeneity and cell-specific dynamics, which is key to understanding many diseases, immunity, development and more. This is in contrast to traditional techniques, that either allowed analysis of a few genes in thousands of individual cells (e.g., in situ hybridization), or the expression profile of thousands of genes, but only on homogenized samples.
Single nuclei RNA sequencing
Single nuclei RNA Sequencing (sNuc-Seq) is a technique which uses isolated nuclei instead of whole cells to profile gene expression in cells which are difficult to isolate. Using droplet microfluidic technology this enables users to profile thousands of nuclei at low cost, in a highly parallel method
How it works
SNuc-seq uses droplet microfluidics to encapsulate single nuclei with uniquely barcoded mRNA capture beads. Following downstream processing and sequencing, the transcriptomes of thousands of single nuclei can be analysed, and unique cell types identified.
sNuc-seq uses droplet microfluidics to encapsulate single nuclei with uniquely barcoded mRNA capture beads. Following downstream processing and sequencing, the transcriptomes of thousands of single nuclei can be analysed, and unique cell types identified.
Nuclei can usually be rapidly and easily isolated from frozen tissues, without the extended incubation and processing required for isolating single cells. The rapid isolation of nuclei from complex tissue will allow researchers to acquire transcriptomes that are relatively unperturbed by the isolation procedure.
RNAdia 2.0 reagent kit for cells and nuclei transcriptomics
One solution to take you from cells to data.
With the RNAdia 2.0 kit for the Nadia Instrument, gain access to a hassle free, streamlined workflow, where you can create single cell and single nuclei transcriptome libraries and analyse them, all in one place.
Single cell research doesn’t need to be a hassle.
The Nadia Instrument takes scRNA-Seq and sNuc-Seq to the next level by using automation and flexibility to generate high quality reproducible single cell data at high throughput to assay tens of thousands of cells or nuclei. Together with the RNAdia 2.0 reagent kit and its streamlined workflow, this becomes a low cost, high quality, complete workflow for single cell sequencing and single nuclei sequencing.
Alongside RNAdia 2.0 we now offer our free Droplet Analyzer Software and the Bioinformatics service add on, providing you with all the tools required to answer your research questions.
RNAdia 2.0 key features
Low cost reagents
Extremely cost effective when compared to other products in the field
Streamlined workflow
Go from cell encapsulation to amplified cDNA libraries in as little as 6h, allowing you to concentrate on cell preparation if necessary
End-to-end workflow
With our RNAdia 2.0 kit and Bioinformatics service you have everything you need to go from cells to data
Very high sample compatibility
Our microfluidic chip design is compatible with a broad range of cell sizes
Capabilities
Tumour heterogeneity
and clonal evolution
Complex tissues with
diverse cell compositions
High resolution identification of cell types and marker genes
Products and services for the complete RNAdia 2.0 workflow
Nadia
Take your research to the next level with Nadia- our microfluidic technology and pre-optimized processes, your analysis and discovery
- Select microfluidic steps defined by us
- Deploy up to 8 samples in parallel
- Measure- analyze your results; answer your research question
Bioinformatics service
Alongside our RNAdia 2.0 kits you can purchase vouchers for our Bioinformatics service, entitling you to data analysis services as part of a bundle with the RNAdia 2.0 kit. Voucher codes match the size of the kit, so an 8-sample kit entitles you to 8 vouchers, meaning that you will get 8 samples analyzed.
What’s included?
- Pre-processing: quality control, filtering, count matrix
- Preliminary clustering
- Report: MultiQC summary, Seurat object, UMAP and/or t-SNE, and comments on your data
Droplet Analyzer software
Detect and analyze hydrogel scaffolds and aqueous droplets generated with microfluidics technology.Free, easy to use software designed to help researchers accurately calculate droplet size, bead capture frequency or multiplet proportion with ease.
40 μm FACS Sortable Click Chip
High quality small droplets are
just a click away
Easy to use microfluidic glass chips for
FACS sortable hydrogel scaffolds and droplet genomics
Using our world-leading expertise we have created a microfluidic solution specifically designed to make small droplets, in order to provide optimal conditions for hydrogel scaffold production and increase the efficiency of your droplet genomics workflows.
40 µm FACS Sortable Click Chip features:
- Compatible: smaller channels enable the production of hydrogel scaffolds that are compatible downstream with standard FACS instruments
- Sort by external cellular environment, without the need for droplet sorting
- Easy to use: regardless of the level of your microfluidic expertise
- Flexible: explore droplet genomics, or hydrogel scaffold production
- Reusable: cost saving, multiple use glass microfluidic chips
Learn more about RNAdia
Download
RNAdia 2.0 for cells app note
Watch
The RNAdia 2.0 intro video
Download
Download RNAdia 2.0 for nuclei application note