Dolomite Bio has launched a new Injection Valve and Sample Loop for single cell RNA sequencing workflows. This option enables straightforward, gentle introduction of evenly distributed mRNA capture beads into the company’s RNA-Seq System for individual encapsulation of cells.
Researchers at The Institute of Cancer Research (ICR), London, are taking advantage of the single cell encapsulation capabilities of Dolomite Bio’s Single Cell RNA-Seq System to investigate resistance mechanisms in prostate cancer. Karolina Nowakowska, a PhD student at The Institute of Cancer Research, explained: “Our team is focused on studying treatment resistance in prostate cancer patients, looking at the biochemical mechanisms responsible.
Dolomite Bio’s Single Cell RNA-Seq System is helping researchers at the University of Helsinki to investigate autoimmune diseases. Focusing on gastrointestinal conditions – such as coeliac disease and inflammatory bowel disease – the Molecular Genetics of Immunological Diseases group is using the system to study T-cell activation and response at the single cell level.
Dolomite Bio has developed the Single Cell RNA-Seq System for efficient, high throughput single cell transcriptomics. This compact, scalable system enables rapid, reproducible droplet encapsulation of individual cells, allowing up to 10,000 single cell libraries to be generated in one 15-minute run.
The creation of non-natural nucleic acid molecules is of great interest to synthetic biology, allowing the development of artificial nucleotides for a wide range of applications. Because these nucleotides are not found in nature, naturally occurring polymerases struggle to process them, limiting researchers’ ability to form complex polymers. One approach to overcome this issue is the directed evolution of polymerases derived from Archaea and other extremophiles, but this technique is both labor intensive and difficult to perform on a large scale. Researchers at Arizona State University’s Biodesign Institute have turned to microfluidics to allow rapid sorting and screening of novel polymerases.